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Polymorphisms within theHLA-DR3haplotypes

✍ Scribed by Ronald Bontrop; Marcel Tilanus; Marlies Mikulski; Marja Eggermond; Annemarie Termijtelen; Marius Giphart

Book ID
104746143
Publisher
Springer-Verlag
Year
1986
Tongue
English
Weight
540 KB
Volume
23
Category
Article
ISSN
0093-7711

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✦ Synopsis

HLA-DR molecules were isolated from eight different HLA-DR3 homozygous B-cell lines by immunoprecipitation with monoclonal antibodies, and they were subsequently analyzed by two-dimensional gel electrophoresis. We found that HLA-DR3 homozygous Bcell lines of consanguineous origin express two types of HLA-DR molecules. One type of HLA-DR molecule was present in all the cell lines tested, whereas the second DR molecule appears to be polymorphic. DNA isolated from the different HLA-DR3 homozygous cell lines was studied by Southern blot analysis to determine whether any DRfl restriction fragment length polymorphism could be observed. Polymorphisms detected at both the product and genomic level have been compared to each other, and their relations to the serological (HLA-DR) and cellular (HLA-D and LB-Q1) typing data will be discussed.

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