Polymorphism of human salivary amylase
โ Scribed by Kamala Soyza
- Book ID
- 104705712
- Publisher
- Springer
- Year
- 1978
- Tongue
- English
- Weight
- 324 KB
- Volume
- 45
- Category
- Article
- ISSN
- 0340-6717
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โฆ Synopsis
Variation of human salivary amylase isoenzymes was investigated by isoelectric focusing on thin-layer polyacrylamide gel. Preliminary studies indicate a genetic polymorphism.
๐ SIMILAR VOLUMES
The plasmid clone which contains human salivary amylase cDNA was used to detect restriction fragment length polymorphisms (RFLPs). After double digestion with Pst 1 and Bam H1, a polymorphism with two alleles was observed. In Japanese, frequencies of these alleles, tentatively called 5.7 kb and 6.5
A radioimmunoassay (RIA) for human salivary amylase was developed. Human salivary amylase was purified from parotid saliva by a combination of Sephadex gel filtration and cation exchange chromatography. Purified salivary amylase was used both as the standard antigen and for the generation of Y-label