Polymerization mechanism of polypeptide chain aggregation

✍ Scribed by Margaret A. Speed; Jonathan King; Daniel I. C. Wang

• Publisher: John Wiley and Sons
• Year: 1997
• Tongue: English
• Weight: 548 KB
• Volume: 54
• Category: Article
• ISSN: 0006-3592
• DOI: 10.1002/(sici)1097-0290(19970520)54:4<333::aid-bit6>3.0.co;2-l

✦ Synopsis

The misfolding of polypeptide chains and ag-and Georgiou, 1991), currently each new protein regregation into the insoluble inclusion body state is a seriquires unique folding optimization on a case-by-case ous problem for biotechnology and biomedical research.

basis. An understanding of the polymerization mecha-Developing a rational strategy to control aggregation renism of aggregation may lead to the rational design of quires understanding the mechanism of polymerization.

such folding strategies.

We investigated the in vitro aggregation of P22 tailspike polypeptide chains by classical light scattering, nondena-Unlike crystallization and self-assembly processes, agturing gel electrophoresis, two-dimensional polyacrylgregation involves the association of thermolabile foldamide gel electrophoresis (PAGE), and computer simulaing intermediates rather than the stable native protein tions. The aggregation of polypeptide chains during