Polymerase Resistance to Polymerase Chain Reaction Inhibitors in Bone
โ Scribed by Kenneth D. Eilert; David R. Foran
- Book ID
- 109280530
- Publisher
- John Wiley and Sons
- Year
- 2009
- Tongue
- English
- Weight
- 120 KB
- Volume
- 54
- Category
- Article
- ISSN
- 0022-1198
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A variety of methods have been developed to amplify DNA and RNA. These methods vary in their susceptibility to evolve new molecular species differing from the starting template. PCR is exceptionally resistant to in vitro evolution, whereas methods such as Q[3 replicase and 3SR are much less robust.
This paper is an analysis of a model of the regeneration of damaged DNA during the polymerase chain reaction (PCR) made to estimate the degree to which recombination between similar but not identical sequences ("jumping PCR") compromises the results obtained with the technique. The analysis is appli