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Polymerase Chain Reaction for Diagnosis ofM. tuberculosis:Comparison of Simple Boiling and a Conventional Method for DNA Extraction

✍ Scribed by Benoosh Afghani; Harris R. Stutman

Book ID
112238095
Publisher
Elsevier Science
Year
1996
Tongue
English
Weight
189 KB
Volume
57
Category
Article
ISSN
1077-3150

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tage with acid quenching is that it is important to keep pH low during all subsequent analyses. Thus, many proteases will digest only above neutral pH, making it difficult to map the disulfide bonds in the folding intermediates using classical peptide mapping. If, on the other hand, 2-VP is added to