A Single-Step, Eco-Friendly Method to Extract DNA from Mycobacterium tuberculosis for Polymerase Chain Reaction

✍ Scribed by R.Ajay Kumar; R. Indulakshmi; Sathish Mundayoor

• Publisher: Elsevier Science
• Year: 2000
• Tongue: English
• Weight: 53 KB
• Volume: 286
• Category: Article
• ISSN: 0003-2697
• DOI: 10.1006/abio.2000.4814

✦ Synopsis

tage with acid quenching is that it is important to keep pH low during all subsequent analyses. Thus, many proteases will digest only above neutral pH, making it difficult to map the disulfide bonds in the folding intermediates using classical peptide mapping. If, on the other hand, 2-VP is added to the acid-quenched folding reaction and pH is brought up to a value where the modification reaction will occur much faster than thiol oxidation and reshuffling, it should be possible to combine the advantages of the two different quenching procedures.

Acknowledgments. We thank Morten Kielland-Brandt and Henrik Ostergaard for reading the manuscript. K.L.L. was supported by a scholarship from the University of Copenhagen.