✦ LIBER ✦

Polymerase chain reaction for detection of cytokine gene expression

✍ Scribed by Anne O'Garra; Paulo Vieira

Publisher: Elsevier Science
Year: 1992
Tongue: English
Weight: 740 KB
Volume: 4
Category: Article
ISSN: 0952-7915
DOI: 10.1016/0952-7915(92)90016-8

No coin nor oath required. For personal study only.

✦ Synopsis

A powerful method to amplify reverse-transcribed RNA, the polymerase chain reaction can be used to measure cytokine gene transcription in a small number of cells, or in cases where there is low mRNA copy number. This technique may be used to obtain qualitative or quantitative determinations of cytokine gene expression. In this review we discuss the various strategies recently described for the evaluation of cytokine expression using the polymerase chain reaction.

📜 SIMILAR VOLUMES

Measurement of Gene Expression by Multip

Measurement of Gene Expression by Multiplex Competitive Polymerase Chain Reaction

✍ M.J. Apostolakos; W.H.T. Schuermann; M.W. Frampton; M.J. Utell; J.C. Willey 📂 Article 📅 1993 🏛 Elsevier Science 🌐 English ⚖ 715 KB

The polymerase chain reaction for detect

The polymerase chain reaction for detection of T-cell antigen receptor expression

✍ Michael A. Panzara; Jorge R. Oksenberg; Lawrence Steinman 📂 Article 📅 1992 🏛 Elsevier Science 🌐 English ⚖ 807 KB

Applications of the polymerase chain reaction have revolutionized the field of immunogenetics, particularly in studies of human leukocyte antigen class II polymorphism, and more recently in the analysis of T-cell receptor usage. However, the enormous diversity and variability of the T-cell receptor

Utility of the polymerase chain reaction

Utility of the polymerase chain reaction in detection of gene expression in drug-resistant human tumors

✍ Kevin J. Scanlon; Mohammed Kashani-Sabet 📂 Article 📅 1989 🏛 John Wiley and Sons 🌐 English ⚖ 659 KB

Recently, an enzymatic amplification method, the polymerase chain reaction (PCR), was modified to amplify a sequence of a drug resistance gene. The PCR assay can confirm data achieved by conventional molecular biology techniques, while requiring less time and fewer patient cells. It can be quantitat

Modifying Differential Display Polymeras

Modifying Differential Display Polymerase Chain Reaction to Detect Relative Changes in Gene Expression Profiles

✍ Daya G. Ranamukhaarachchi; Mangalathu S. Rajeevan; Suzanne D. Vernon; Elizabeth 📂 Article 📅 2002 🏛 Elsevier Science 🌐 English ⚖ 189 KB

A Polymerase Chain Reaction-Based Method

A Polymerase Chain Reaction-Based Method for Detection and Quantification of Reporter Gene Expression in Transient Transfection Assays

✍ M.J. Morales; D.I. Gottlieb 📂 Article 📅 1993 🏛 Elsevier Science 🌐 English ⚖ 583 KB

Transcription in higher eukaryotes is often studied by the use of transient transfection assays. These experiments are performed by cloning putative cis-acting transcriptional elements (i.e., a promoter or enhancer) with a reporter gene that codes for a protein not expressed by the target cells. Alt

Immunopolymerase Chain Reaction Using Re

Immunopolymerase Chain Reaction Using Real-Time Polymerase Chain Reaction for Detection

✍ Paul W. Sims; Mark Vasser; Wai Lee Wong; P.Mickey Williams; Y.Gloria Meng 📂 Article 📅 2000 🏛 Elsevier Science 🌐 English ⚖ 56 KB

All articles available online at http://www.idealibrary.com on FIG. 3. Correlation of VEGF concentrations in 40 normal human serum samples measured by fluorometric ELISA ( y axis) and immuno-PCR ( x axis) ( y ϭ 0.76 x Ϫ 7 pg/ml, correlation coefficient ϭ 0.83, P Ͻ 0.0001).