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Polymerase chain reaction-based gene disruption in Trypanosoma brucei

✍ Scribed by Anne Gaud; Mark Carrington; Jacques Deshusses; Dominique R.G Schaller


Book ID
114146270
Publisher
Elsevier Science
Year
1997
Tongue
English
Weight
120 KB
Volume
87
Category
Article
ISSN
0166-6851

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Gene disruption is an important method for genetic analysis in Saccharomyces cerevisiae. We have designed a polymerase chain reaction-directed gene disruption cassette that allows rapid disruption of genes in S. cerevisiae without previously cloning them. In addition, this cassette allows recycling