pMPY-ZAP: A reusable polymerase chain reaction-directed gene disruption cassette forSaccharomyces cerevisiae

✍ Scribed by Schneider, B. L.; Steiner, B.; Seufert, W.; Futcher, A. B.

• Book ID: 101225571
• Publisher: John Wiley and Sons
• Year: 1996
• Tongue: English
• Weight: 424 KB
• Volume: 12
• Category: Article
• ISSN: 0749-503X
• DOI: 10.1002/(sici)1097-0061(199602)12:2<129::aid-yea891>3.0.co;2-o

✦ Synopsis

Gene disruption is an important method for genetic analysis in Saccharomyces cerevisiae. We have designed a polymerase chain reaction-directed gene disruption cassette that allows rapid disruption of genes in S. cerevisiae without previously cloning them. In addition, this cassette allows recycling of URA3, generating gene disruptions without the permanent loss of the ura3 marker. An indefinite number of disruptions can therefore be made in the same strain.