✦ LIBER ✦

Polymer supported dna synthesis using hydroxybenzotriazole activated phosphotriester intermediates

✍ Scribed by J.E. Marugg; L.W. McLaughlin; N. Piel; M. Tromp; G.A. van der Marel; J.H. van Boom

Publisher: Elsevier Science
Year: 1983
Tongue: French
Weight: 242 KB
Volume: 24
Category: Article
ISSN: 0040-4039
DOI: 10.1016/s0040-4039(00)88244-1

No coin nor oath required. For personal study only.

✦ Synopsis

In the present method appropriate N-protected-S'-0-(4,4'-dimethoxytrityl)-2'-deoxynucleosides are phosphorylated with the reagent 2-chlorophenyl-O,O-bis(l-benzotriazolyl)-phosphate and the 3'-phosphorylated intermediates thus obtained are used directly to elongate the polymer support Kieselgur-polydimethylacrylamide.

According to this procedure, we prepared two DNA fragments: one decamer and a heptadecamer.

📜 SIMILAR VOLUMES

Synthesis of DNA on a solid support by t

Synthesis of DNA on a solid support by the hydroxybenzotriazole phosphotriester approach

✍ J. E. Marugg; G. A. van der Morel; E. de Vroom; D. Bosch; J. H. van Boom 📂 Article 📅 2010 🏛 Elsevier Science 🌐 English ⚖ 159 KB

## Abstract Application of the phosphorylating agent 2‐chlorophenyl‐0,0‐bis(1‐hydroxybenzotriazolyl)‐phosphate enabled us to synthesize DNA fragments (e.g., d‐AGGATCCT and d‐GTTCGG) on a polystyrene solid support:

Asymmetric synthesis by use of polymer-s

Asymmetric synthesis by use of polymer-supported chiral activating agents

✍ Shinichi Itsuno; Jean M.J. Fréchet 📂 Article 📅 1989 🏛 Elsevier Science ⚖ 165 KB

ChemInform Abstract: Direct Immobilizati

ChemInform Abstract: Direct Immobilization of Oligonucleotides on Polymer Supports: Use of FMOC Derivatives in DNA/RNA Synthesis — Methods for Using Immobilized Oligonucleotides.

✍ G. Alvarado Urbina; H. Schirmeister-Tichy; J. Schernthaner; A. Taralp; W. Pfleid 📂 Article 📅 2010 🏛 John Wiley and Sons ⚖ 27 KB 👁 2 views

Polymer Supported Carbodiimide Strategy

Polymer Supported Carbodiimide Strategy for the Synthesis of N-Acylated Derivatives of Deoxy- and Ribo-Purinenucleosides Using Active Esters.

✍ Snehlata Tripathi; Krishna Misra; Yogesh S. Sanghvi 📂 Article 📅 2006 🏛 John Wiley and Sons ⚖ 14 KB

Hormone-Receptor Interactions. Synthesis

Hormone-Receptor Interactions. Synthesis of a biologically active cysteinyl-angiotensin derivative and its use for the preparation of spin-labelled and polymer-supported molecules

✍ Hans Jörg Möschler; Robert Schwyzer 📂 Article 📅 1974 🏛 John Wiley and Sons 🌐 German ⚖ 530 KB

## Abstract (N‐t‐Butoxycarbonyl‐S‐acetamido‐methyl‐L‐cysteinyl)‐[1‐ asparagine, 5‐valine]‐angiotensin II (**2**) was prepared from [1‐asparagine, 5‐valine]‥angiotensin II (**1**). With respect to enhancement of rat blood pressure, it is a full agonist displaying about 20–25% of the potency of **1**