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Polymer-based monolithic microcolumns for hydrophobic interaction chromatography of proteins

✍ Scribed by Petrus Hemström; Anna Nordborg; Knut Irgum; Frantisek Svec; Jean M. J. Fréchet

Book ID
102925645
Publisher
John Wiley and Sons
Year
2006
Tongue
English
Weight
1000 KB
Volume
29
Category
Article
ISSN
1615-9306

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✦ Synopsis

Abstract

Monolithic capillary columns for hydrophobic interaction chromatography (HIC) have been prepared by thermally initiated, single‐step in situ polymerization of mixtures of monovinyl monomers including butyl methacrylate and/or 2‐hydroxyethyl methacrylate, with a divinyl crosslinker glycerol dimethacrylate or 1,4‐butanediol dimethacrylate using two different porogen systems. Two porogenic solvent mixtures were used; one “hydrophilic”, consisting of water, butanediol, and propanol, and one “hydrophobic,” comprising dodecanol and cyclohexanol. The porous structures of the monoliths were characterized and their performance was demonstrated with a separation of a mixture of myoglobin, ribonuclease A, and lysozyme under conditions typical of HIC.

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## Abstract Silica monoliths in affinity microcolumns were tested for the high‐throughput analysis of drug–protein interactions. HSA was used as a model protein for this work, while carbamazepine and __R__‐warfarin were used as model analytes. A comparison of HSA silica monoliths of various lengths