✦ LIBER ✦

Polyethenoadenosine Phosphate as a Fluorogenic Substrate for Barnase

✍ Scribed by P.C. Fitzgerald; R.W. Hartley

Publisher: Elsevier Science
Year: 1993
Tongue: English
Weight: 327 KB
Volume: 214
Category: Article
ISSN: 0003-2697
DOI: 10.1006/abio.1993.1536

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✦ Synopsis

Polyethenoadenosine phosphate is rapidly hydrolyzed by barnase with an accompanying large increase in fluorescence. At pH 8, 0.2 M ammonium acetate, the initial rate of fluorescence increase is proportional to barnase concentration and thus provides an excellent quantitative assay for its activity. The preparation and use of this substrate in the barnase assay is presented in detail.

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