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Polyester copolymer scaffolds enhance expression of bone markers in osteoblast-like cells

✍ Scribed by Shaza Bushra Idris; Kristina Arvidson; Peter Plikk; Salah Ibrahim; Anna Finne-Wistrand; Ann-Christine Albertsson; Anne Isine Bolstad; Kamal Mustafa

Publisher
John Wiley and Sons
Year
2010
Tongue
English
Weight
559 KB
Volume
9999A
Category
Article
ISSN
1549-3296

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✦ Synopsis

Abstract

In tissue engineering, the resorbable aliphatic polyester poly(L‐lactide) (PLLA) is used as scaffolds in bone regeneration. Copolymers of poly(L‐lactide)‐co‐(ε‐caprolactone) [poly(LLA‐co‐CL)] and poly(L‐lactide)‐co‐(1,5‐dioxepan‐2‐one) [poly(LLA‐co‐DXO)], with superior mechanical properties to PLLA, have been developed to be used as scaffolds, but the influence on the osteogenic potential is unclear. This in vitro study of test scaffolds of poly(LLA‐co‐CL) and poly(LLA‐co‐DXO) using PLLA scaffolds as a control demonstrates the attachment and proliferation of human osteoblast‐like cells (HOB) as measured by SEM and a methylthiazol tetrazolium (MTT) colorimetric assay, and the progression of HOB osteogenesis for up to 3 weeks; expressed as synthesis of the osteoblast differentiation markers: collagen type 1 (Col 1), alkaline phosphatase, bone sialoprotein, osteocalcin (OC), osteopontin and runt related gene 2 (Runx2). Surface analysis disclosed excellent surface attachment, spread and penetration of the cells into the pores of the test scaffolds compared to the PLLA. MTT results indicated that test scaffolds enhanced the proliferation of HOBs. Cells grown on the test scaffolds demonstrated higher synthesis of Col 1 and OC and also increased bone markers mRNA expression. Compared to scaffolds of PLLA, the poly(LLA‐co‐CL) and poly(LLA‐co‐DXO) scaffolds enhanced attachment, proliferation, and expression of osteogenic markers by HOBs in vitro. Therefore, these scaffolds might be appropriate carriers for bone engineering. © 2010 Wiley Periodicals, Inc. J Biomed Mater Res Part A, 2010

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