Polyclonal and monoclonal antibodies specific to the chrysanthemic acid moiety of pyrethroid insecticides

✍ Scribed by Miyake, Shiro; Beppu, Rie; Yamaguchi, Yuki; Kaneko, Hideo; Ohkawa, Hideo

• Publisher: John Wiley and Sons
• Year: 1998
• Tongue: English
• Weight: 231 KB
• Volume: 54
• Category: Article
• ISSN: 1526-498X
• DOI: 10.1002/(sici)1096-9063(1998110)54:3<189::aid-ps812>3.0.co;2-a

✦ Synopsis

Two polyclonal and two monoclonal antibodies raised against chrysanthemic acid (CAA) were prepared for ELISA of pyrethroid insecticides with the common CAA moiety. The monoclonal antibody KCA226 showed the highest reactivity towards allethrin among the antibodies in C-ELISA, although KCA226 was more sensitive to higher concentrations of methanol than the polyclonal antibodies in ELISA. KCA226 was reacted linearly with allethrin at concentrations ranging from 1 to 10 lg litre~1 in 0É5% methanol and from 300 to 10 000 lg litre~1 in 30% methanol. KCA226 reacted speciÐcally with the pyrethroid insecticides with the CAA moiety but was much less reactive with CAA itself. These results suggest that C-ELISA based on KCA226 is useful for the assay of pyrethroid residues with the common CAA moiety.

1998 Society of ( Chemical Industry