Polyclonal and monoclonal antibodies for the specific detection of the herbicide acifluorfen and related compounds

✍ Scribed by Miyake, Shiro; Hayashi, Akiko; Kita, Hiroshi; Ohkawa, Hideo

• Publisher: John Wiley and Sons
• Year: 1997
• Tongue: English
• Weight: 257 KB
• Volume: 51
• Category: Article
• ISSN: 1526-498X
• DOI: 10.1002/(sici)1096-9063(199709)51:1<49::aid-ps571>3.0.co;2-y

✦ Synopsis

Polyclonal and monoclonal antibodies reactive with aciÑuorfen (AF) were prepared by the immunization of, respectively, rabbits and mice with AFbovine serum albumin conjugates. The reactivities of polyclonal antibody and three monoclonal antibodies (AF 9-1, AF 51-5 and AF 75-144) were examined in an indirect competitive enzyme-linked immunosorbent assay (C-ELISA). The polyclonal antibody reacted with AF at concentrations of 1É5 to 800 kg litre~1, while the monoclonal antibodies reacted with AF at concentrations of 3 to 24 kg litre~1 for AF 9-1, 1É5 to 12 kg litre~1 for AF 51-5 and 12 to 48 kg litre~1 for AF 75-144. In the presence of up to 40% methanol in C-ELISA, the monoclonal antibodies, particularly AF 75-144, were less a †ected in their reactivities with AF than was the polyclonal antibody. Moreover AF 9-1 and AF 51-5 speciÐcally reacted with aciÑuorfen-methyl and oxyÑuorfen, while AF 75-144 reacted with chlornitrofen which did not react with the other antibodies. These results indicated that the antibodies are useful for the assay of AF and its related compounds.