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Polarographic Reduction of Cystine and of Dithiodiglycolic Acid in the Presence of Iron

✍ Scribed by Kolthoff, I. M.; Stricks, W.; Tanaka, Nobuyuki

Book ID
127186136
Publisher
American Chemical Society
Year
1955
Tongue
English
Weight
536 KB
Volume
77
Category
Article
ISSN
0002-7863

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Cysteine and half-cystine in proteins and peptides can be determined by acid hydrolysis in the presence of dithiodiglycolic acid, derivatization with phenyl isothiocyanate, and HPLC separation of the phenylthiocarbamyl amino acids. The determination requires no additional sample handling, oxidation,