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Point Mutation Genotyping Based on Melting-Point Difference without Fluorescent Probes

โœ Scribed by Xuan Meng; Jianhua Huang; Dr. Xiangxian Meng; Yanqiong Wu; Qiuhua Mo


Book ID
102096074
Publisher
John Wiley and Sons
Year
2010
Tongue
English
Weight
63 KB
Volume
28
Category
Article
ISSN
0256-7660

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โœ Philip S. Bernard; Marla J. Lay; Carl T. Wittwer ๐Ÿ“‚ Article ๐Ÿ“… 1998 ๐Ÿ› Elsevier Science ๐ŸŒ English โš– 131 KB

A microvolume fluorimeter integrated with a rapid thermal cycler allows both amplification and point mutation detection from genomic DNA in approximately 30 min. This homogeneous method combines rapid cycle DNA amplification with allele-specific fluorescent probe melting profiles for product genotyp