Addition of prostaglandin E1 (PGE1) to quiescent cultures of Swiss 3T3 cells rapidly elevates the intracellular levels of cAMP and increases the activity of adenylate cyclase in particulate fractions of these cells. In the presence of insulin, PGE1 stimulates the reinitiation of DNA synthesis. Both
โฆ LIBER โฆ
Platelet-derived growth factor elicits cyclic AMP accumulation in swiss 3T3 cells: Role of prostaglandin production
โ Scribed by Rozengurt, Enrique; Stroobant, Paul; Waterfield, Michael D.; Deuel, Thomas F.; Keehan, Margaret
- Book ID
- 123556375
- Publisher
- Elsevier Science
- Year
- 1983
- Tongue
- English
- Weight
- 981 KB
- Volume
- 34
- Category
- Article
- ISSN
- 0092-8674
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Incubation of Swiss mouse 3T3 cells at 37ยฐC with bovine brain-derived growth factor (BDGF) decreased the cell surface '251-EGF binding activity of these cells by 70-80%. This down-modulation of the EGF receptor by BDGF was time, temperature, and dose dependent. Scatchard plot analysis indicated that