𝔖 Bobbio Scriptorium
✦   LIBER   ✦

Plasmids expressing the herpes simplex virus thymidine kinase gene in mammalian and bacterial cells

✍ Scribed by Strauss, Michael ;Kiessling, Udo ;Kähler, Ruth

Publisher
Springer
Year
1983
Tongue
English
Weight
479 KB
Volume
191
Category
Article
ISSN
0026-8925

No coin nor oath required. For personal study only.

✦ Synopsis

Two plasmids containing either the complete thymidine kinase gene of Herpes simplex virus type I (pSK2) or the gene without the remote control sequence (pSK1) just behind the lac promoter and the first codons of the lacZ gene were constructed. Both plasmids efficiently transform mouse Ltk- cells as well as E. coli tk- cells to the Tk+ phenotype and are well suited for plasmid rescue from transformed mouse cells by direct functional selection for tk expression using a tk- mutant of E. coli C600.

📜 SIMILAR VOLUMES

Ganciclovir nucleotides accumulate in mi
Ganciclovir nucleotides accumulate in mitochondria of rat liver cells expressing the herpes simplex virus thymidine kinase gene
✍ Marjolijn M. van der Eb; Sacha B. Geutskens; André B. P. van Kuilenburg; Henk va 📂 Article 📅 2003 🏛 John Wiley and Sons 🌐 English ⚖ 424 KB

## Abstract ## Background Ganciclovir exhibits broad‐spectrum activity against DNA viruses such as cytomegaloviruses, herpes simplex viruses, varicella‐zoster virus, Epstein‐Barr virus and human herpes virus‐6. Ganciclovir is widely applied for anti‐herpetic treatment, cytomegalovirus prophylaxis

Sites of integration of herpes simplex v
Sites of integration of herpes simplex virus type-2 thymidine kinase gene in human chromosomes
✍ Saul Kit; Yael Teitz; Marion Hazen; Hamida Qavi 📂 Article 📅 1979 🏛 John Wiley and Sons 🌐 French ⚖ 767 KB

## Abstract Isozyme analyses have been carried out to investigate the sites of integration of the herpes simplex type 2 (HSV‐2) thymidine kinase (TK) gene in biochemically transformed human [HeLa(BU25)/HSV‐2–6 Cl 4] cells. Extracts were prepared from He La (BU25)/HSV‐2–6 Cl 4 cells and from human—m

Ectopic expression of herpes simplex vir
Ectopic expression of herpes simplex virus-thymidine kinase gene in human non-small cell lung cancer cells conferred caspase-activated apoptosis sensitized by ganciclovir
✍ Chien-Chih Chiu; Yuan-Lin Kang; Tsung-Hsiang Yang; Chih-Hsin Huang; Kang Fang 📂 Article 📅 2002 🏛 John Wiley and Sons 🌐 French ⚖ 689 KB

Human non-small cell lung cancer (NSCLC) cells were transfected with recombinant prodrug herpes simplex virus type I thymidine kinase (HSV-tk) cDNA, and the selected clones underwent apoptosis in response to induction by antiviral ganciclovir (GCV). The efficiency of GCV-induced growth inhibition an