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Plasmid Stabilization of an Escherichia coli Culture through Cycling

โœ Scribed by M. L. Stephens; C. Christensen; G. Lyberatos

Book ID
114420499
Publisher
American Institute of Chemical Engineers
Year
1992
Tongue
English
Weight
502 KB
Volume
8
Category
Article
ISSN
8756-7938

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Plasmid pTSO118 containing the Escherichia coli origin of replication, oriC, initiated replication simultaneously with the chromosome when temperature-sensitive host cells were synchronized by temperature shifts. Replicating intermediates of the plasmid as well as of the chromosome were isolated fro