𝔖 Bobbio Scriptorium
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Plasmid maintenance in Bacillus subtilis recombination-deficient mutants

✍ Scribed by Alonso, Juan C. ;Viret, Jean-François ;Tailor, Ravindra H.

Book ID
104725124
Publisher
Springer
Year
1987
Tongue
English
Weight
485 KB
Volume
208
Category
Article
ISSN
0026-8925

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✦ Synopsis

An isogenic set of 11 recombination-deficient mutant strains of Bacillus subtilis has been constructed. Whereas plasmid pUB110 is stably maintained in such Rec- cells, the high copy number plasmid pC194 is unstable. Instability in Rec- strains could be mostly attributed to the deleterious effect of the presence of the plasmid on the Rec- cells' growth capability. In part, instability of pC194 derivatives could also be correlated with the presence of an unusually high amount of multimeric DNA molecules.

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