Plasmid maintenance in Bacillus stearothermophilus is strain-dependent
β Scribed by Linda Oskam; Gerard Venema; Sierd Bron
- Book ID
- 109317459
- Publisher
- John Wiley and Sons
- Year
- 1992
- Tongue
- English
- Weight
- 428 KB
- Volume
- 93
- Category
- Article
- ISSN
- 0378-1097
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An isogenic set of 11 recombination-deficient mutant strains of Bacillus subtilis has been constructed. Whereas plasmid pUB110 is stably maintained in such Rec- cells, the high copy number plasmid pC194 is unstable. Instability in Rec- strains could be mostly attributed to the deleterious effect of
## Abstract Bacillus stearothermophilus cells vary the lipid fatty acid composition of cytoplasmic membranes with growth temperature. Spin label studies of such membranes have been interpreted to indicate lateral lipid phase separations at the growth temperature. We have now used freezeβfracture el