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Plasmid DNA transmission through charged ultrafiltration membranes

✍ Scribed by Kimberly Ager; David R. Latulippe; Andrew L. Zydney

Publisher
Elsevier Science
Year
2009
Tongue
English
Weight
341 KB
Volume
344
Category
Article
ISSN
0376-7388

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## Abstract Recent studies have demonstrated the feasibility of using ultrafiltration for the purification of plasmid DNA, but there is still little understanding of the factors governing DNA transmission. Experimental data were obtained for the transmission of a 3.0 kbp supercoiled plasmid DNA thr

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Ultrafiltration membranes having electric charge, made of sulfonated polysulfone, were manufactured. Two types of membrane having charge density of 1.6 and 0.78 meq/g, and designated as Ml and M2, respectively, were used. Rejections of various molecular weight PEG were first measured by using these