๐”– Bobbio Scriptorium
โœฆ   LIBER   โœฆ

Plasmid DNA from methanogenic bacteria

โœ Scribed by Bruno Biavati; Barbara Sgorbati; Domenico Palenzona


Publisher
Springer
Year
1992
Tongue
English
Weight
386 KB
Volume
24
Category
Article
ISSN
0343-8651

No coin nor oath required. For personal study only.


๐Ÿ“œ SIMILAR VOLUMES


A rapid method for the isolation of plas
โœ J. T. Trevors ๐Ÿ“‚ Article ๐Ÿ“… 1984 ๐Ÿ› Springer Netherlands ๐ŸŒ English โš– 286 KB

A rapid method is described for the isolation of plasmid DNA from Escherichia coli and Pseudomonas putida. The effect of heating the cell preparation during plasmid extraction is discussed in relationship to the final plasmid yield.

Transformation of bacteria with plasmid
โœ Stefan Fiedler; Reinhard Wirth ๐Ÿ“‚ Article ๐Ÿ“… 1988 ๐Ÿ› Elsevier Science ๐ŸŒ English โš– 565 KB

The possibility of electric field-mediated transformation ("electroporation") of a gram-positive bacterium (Enterococcus faecalis) and two gram-negative bacteria (Escherichia coli and Pseudomonas putida) with plasmid DNA was investigated. E. faecalis protoplasts could be transformed by electroporati

Release of transforming plasmid and chro
โœ Michael G. Lorenz; Detlef Gerjets; Wilfried Wackernagel ๐Ÿ“‚ Article ๐Ÿ“… 1991 ๐Ÿ› Springer ๐ŸŒ English โš– 843 KB

The release of chromosomal and plasmid DNA from Acinetobacter calcoaceticus and Bacillus subtilis cultivated in minimal medium and broth over a period of 50 h was monitored and related to growth phase, autolysis, DNase production and natural competence. The released DNAs were biologically active in

A simple procedure for large-scale prepa
โœ M. Mukhopadhyay; N.C. Mandal ๐Ÿ“‚ Article ๐Ÿ“… 1983 ๐Ÿ› Elsevier Science ๐ŸŒ English โš– 870 KB

A very simple, inexpensive procedure for preparing pure plasmid DNA from bacteria is described. In this method, lysozyme-induced spheroplasts are made in presence of 833 micrograms/ml of ethidium bromide which are then lysed by a mixture of Brij 58 and sodium deoxycholate, and the lysate is centrifu