Plasma thiols redox status by laser-induced fluorescence capillary electrophoresis
β Scribed by Ciriaco Carru; Luca Deiana; Salvatore Sotgia; Giovanni Mario Pes; Angelo Zinellu
- Publisher
- John Wiley and Sons
- Year
- 2004
- Tongue
- English
- Weight
- 103 KB
- Volume
- 25
- Category
- Article
- ISSN
- 0173-0835
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We present a new analytical method for thiol quantification in plasma, based on the use of capillary electrophoresis (CE) and laser-induced fluorescence (LIF) to analyze 6-iodoacetamidofluorescein derivatives. Quantitative results of homocysteine, glutathione, cysteinylglycine, and cystationine are
## Abstract We describe an ultrarapid capillary electrophoresis with laserβinduced fluorescence (CEβLIF) method for total plasma thiols measurement. Reduced thiols by 10% triβ__n__βbutylphosphine (TBP) were derivatized in 10Β min at room temperature with 5βiodoacetamidofluorescein (5βIAF) as fluores