Plasma total homocysteine and other thiols analyzed by capillary electrophoresis/laser-induced fluorescence detection: Comparison with two other methods
✍ Scribed by Elisabeth Caussé; Pascal Malatray; Raymond Calaf; Philippe Charpiot; Mirande Candito; Christophe Bayle; Pierre Valdiguié; Robert Salvayre; François Couderc
- Publisher
- John Wiley and Sons
- Year
- 2000
- Tongue
- English
- Weight
- 124 KB
- Volume
- 21
- Category
- Article
- ISSN
- 0173-0835
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✦ Synopsis
We present a new analytical method for thiol quantification in plasma, based on the use of capillary electrophoresis (CE) and laser-induced fluorescence (LIF) to analyze 6-iodoacetamidofluorescein derivatives. Quantitative results of homocysteine, glutathione, cysteinylglycine, and cystationine are presented. A comparison of the quantitation of homocysteine in plasma, using high performance liquid chromatography/fluorescence detection and fluorescence polarization immunoassay is proposed. The results indicate that these techniques for plasma total homocysteine (tHcy) determination can be used interchangeably. The major advantage of CE-LIF is that it can quantitate the thiols in one run while keeping the price of consumables reasonable.