✦ LIBER ✦

Plasma membrane—mediated nuclear uptake and chromatin binding of insulin in tumor cell lines

✍ Scribed by Ewa M. Rakowicz-Szulczynska; Danuta Otwiaska; Hilary Koprowski

Book ID: 102945360
Publisher: John Wiley and Sons
Year: 1990
Tongue: English
Weight: 676 KB
Volume: 3
Category: Article
ISSN: 0899-1987
DOI: 10.1002/mc.2940030308

No coin nor oath required. For personal study only.

✦ Synopsis

Abstract

Analysis of different cellular fractions after incubation of SW 948 and SW 707 colorectal carcinoma cells or WM 266‐4 melanoma cells with ^125^I‐insulin revealed the nondegraded hormone in the chromatin of these cells. Nuclear ^125^1‐insulin was bound to specific fragments of EcoRI‐, Haelll‐, and Hincll‐digested chromatin. A 45‐kDa chromatin protein species that binds ^125^I‐insulin was identified. Actinomycin D and cycloheximide inhibited the insulin‐stimulated expression of chromatin receptors. Uptake of ^125^I‐insulin by isolated nuclei occurred only in the presence of plasma membranes. Thus, at least some effects of insulin on target cells can be explained by direct gene regulation instead of “second messenger” action.

📜 SIMILAR VOLUMES

Nuclear uptake of NGF, EGF, PDGF and ins

Nuclear uptake of NGF, EGF, PDGF and insulin, binding to chromatin receptors in tumour cell lines

✍ Rakowicz-Szulczyńska, Ewa M.; Koprowski, Hilary 📂 Article 📅 1987 🏛 Elsevier Science ⚖ 126 KB

Glucagon increases cellular uptake and p

Glucagon increases cellular uptake and plasma membrane binding of L-carnitine in S49 lymphoma cells

✍ Birgit Gustafson; Lennart A. Ransnäs 📂 Article 📅 1998 🏛 Elsevier Science 🌐 English ⚖ 477 KB

Isolation and identification of heteroge

Isolation and identification of heterogeneous nuclear ribonucleoproteins (hnRNP) from purified plasma membranes of human tumour cell lines as albumin-binding proteins

✍ Thomas Fritzsche; Martina Schnölzer; Sabine Fiedler; Martina Weigand; Manfred Wi 📂 Article 📅 2004 🏛 Elsevier Science 🌐 English ⚖ 375 KB

Characterization of Nuclear Retinoic Aci

Characterization of Nuclear Retinoic Acid-Binding Activity in Sensitive Leukemic-Cell Lines - Cell-Specific Uptake of Atra and Rarα Protein Modulation

✍ A. Agadir; M. Cornic; M. Jerome; M.L. Menot; N. Cambier; M.P. Gaub; B. Gourmel; 📂 Article 📅 1995 🏛 Elsevier Science 🌐 English ⚖ 632 KB

Secretion and biological actions of insu

Secretion and biological actions of insulin-like growth factor binding proteins in two human tumor-derived cell lines in vitro

✍ Cecilia Camacho-Hubner; Robert H. McCusker; David R. Clemmons 📂 Article 📅 1991 🏛 John Wiley and Sons 🌐 English ⚖ 1017 KB

The insulin-like growth factors (IGFs) I and II are present in extracellular fluids associated with specific binding proteins (IGFBPs) that can modify their biologic actions. These studies were undertaken to determine which forms of IGFBP are secreted by endometrial carcinoma (HEC-1 B) and breast ca

Estradiol enhances liposome-mediated upt

Estradiol enhances liposome-mediated uptake, preferential nuclear accumulation and functional expression of exogenous genes in MDA-MB231 breast tumor cells

✍ Pramod T. Jain; Prem Seth; David A. Gewirtz 📂 Article 📅 1999 🏛 Elsevier Science 🌐 English ⚖ 892 KB