𝔖 Bobbio Scriptorium
✦   LIBER   ✦

Plasma amino acid analysis by isotope ratio gas chromatography mass spectrometry computer techniques

✍ Scribed by Martin F. Schulman; Fred P. Abramson

Publisher
John Wiley and Sons
Year
1975
Tongue
English
Weight
636 KB
Volume
2
Category
Article
ISSN
1076-5174

No coin nor oath required. For personal study only.

✦ Synopsis

A routine analysis for micro samples of plasma amino acids by a gas chromatography mass spectrometry computer system has been developed. Isotope ratio determination was used as the quantitating technique via multiple internal standards. The speed of the analysis is increased by omitting the ion exchange purification step and its flexibility is maximized by using repetitive scanning rather than selective ion monitoring. This procedure yields excellent precision and accuracy, as demonstrated by the analysis of a known amino acid mixture and of neonatal plasma.

πŸ“œ SIMILAR VOLUMES

Isotope ratio monitoring gas chromatogra
Isotope ratio monitoring gas chromatography/Mass spectrometry of D/H by high temperature conversion isotope ratio mass spectrometry
✍ A. W. Hilkert; C. B. Douthitt; H. J. SchlΓΌter; W. A. Brand πŸ“‚ Article πŸ“… 1999 πŸ› John Wiley and Sons 🌐 English βš– 91 KB πŸ‘ 2 views

Of all the elements, hydrogen has the largest naturally occurring variations in the ratio of its stable isotopes (D/H). It is for this reason that there has been a strong desire to add hydrogen to the list of elements amenable to isotope ratio monitoring gas chromatography/mass spectrometry (irm-GC/

13C Gas Chromatography–Combustion Isotop
13C Gas Chromatography–Combustion Isotope Ratio Mass Spectrometry Analysis of N-Pivaloyl Amino Acid Esters of Tissue and Plasma Samples
✍ Cornelia C. Metges; Maren Daenzer πŸ“‚ Article πŸ“… 2000 πŸ› Elsevier Science 🌐 English βš– 108 KB

## We present the analysis of the stable carbon isotope compositions of 14 individual N-pivaloyl-isopropyl (NPP) amino acid esters by gas chromatography-combustion isotope ratio mass spectrometry (GC-C-IRMS). The mean reproducibility of derivatization procedure and GC-C-IRMS analysis was 0.45‰ (ra

Determination of (13C)urea enrichment by
Determination of (13C)urea enrichment by gas chromatography/mass spectrometry and gas chromatography/isotope ratio mass spectrometry
✍ Michel Beylot; France David; Yadh Khalfallah; Sylvie Normand; Valerie Large; Hen πŸ“‚ Article πŸ“… 1994 πŸ› John Wiley and Sons 🌐 English βš– 354 KB πŸ‘ 2 views

We present gas chromatographic/mass spectrometric and gas chromatographic/isotope ratio mass spectrometric assays of the 13C enrichment of plasma urea converted to its dimethylaminomethylene derivative. The limits of sensitivity of the two techniques are 0.2% and 0.02%, respectively. The techniques

Measurement of deuterium incorporation i
Measurement of deuterium incorporation into fatty acids by gas chromatography/isotope ratio mass spectrometry
✍ C. M. Scrimgeour; I. S. Begley; M. L. Thomason πŸ“‚ Article πŸ“… 1999 πŸ› John Wiley and Sons 🌐 English βš– 47 KB πŸ‘ 2 views

A continuous-flow pyrolysis interface for gas chromatography/isotope ratio mass spectrometry (GC/IRMS) has been developed for 2 H analysis of individual organic compounds. Pyrolysis of gas chromatographically separated fatty acid methyl esters is performed at 1200 Β°C in an unpacked alumina tube to c