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Plantlet regeneration from encapsulated somatic embryos of hybridSolanum melongenaL

✍ Scribed by P. V. Lakshmana Rao; B. Singh

Publisher
Springer
Year
1991
Tongue
English
Weight
985 KB
Volume
10
Category
Article
ISSN
0721-7714

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✦ Synopsis

High frequency somatic embryogenesis was induced from leaf expiants of F1 hybrid Solanum melongena L. on Murashige and Skoog's medium supplemented with 8.0 mg/1 NAA and 0.1 mg/1 Kn. The somatic embryos were encapsulated in various concentrations (2-6%) of sodium alginate and complexed with calcium chloride (25-100mM): 3% sodium alginate and 75 mM calcium chloride were found to be optimal for encapsulation. The encapsulated somatic embryos were transferred to various conversion media in vitro and in vivo. The frequency of plantlet regeneration varied from 27.0-49.7% in vitro and 2.0-4.5% in vivo.

πŸ“œ SIMILAR VOLUMES

Direct somatic embryogenesis and plantle
Direct somatic embryogenesis and plantlet regeneration from cotyledonary leaves of safflower
✍ A. K. A. Mandal; A. K. Chatterji; S. Dutta Gupta πŸ“‚ Article πŸ“… 1995 πŸ› Springer 🌐 English βš– 395 KB

Somatic embryos were induced directly on adaxial surface of cotyledonary leaves within 8-10 days of culture on Murashige and Skoog medium containing 5.37 to 10.74 laM 1 -napthaleneacetic acid and 2.22 p.M benzyl adenine. Germinated embryos with shoot axes developed into complete plants after transfe