Plantlet formation from callus and shoot-tip culture ofHelianthus annuus(L.)

✍ Scribed by Maria Carla Lupi; Andrea Bennici; Francesco Locci; Delio Gennai

• Publisher: Springer
• Year: 1987
• Tongue: English
• Weight: 688 KB
• Volume: 11
• Category: Article
• ISSN: 0167-6857
• DOI: 10.1007/bf00036575

✦ Synopsis

A method for callus induction, adventitious bud regeneration, shoot multiplication and rooting of in vitro formed shoots of Helianthus annuus L. var. Argentario is described. Hypocotyl and cotyledon explants formed callus on medium containing 2 mg 1 ~ naphthalene acetic acid and 0.5 mgl ~ benzyladenine. Adventitious buds were formed on hypocotyl segments on medium containing 0.5 2 mg 1 -z benzyladenine. The optimal level of sucrose concentration for shoot regeneration from hypocotyls was 1.5%. Multiplication from shoot apices was promoted by kinetin (2mgl -~) plus gibberellic acid (5mg1-~), benzyladenine (2mgl -~) plus gihberellic acid (10mgl t) or at lower frequency by benzyladenine (1 mgl-~). A general feature of the plantlets formed in vitro was the "precocious flowering".