Embryogenic callus cultures of ginger were induced from young leaf segments taken from in vitro shoot cultures. Among the four auxins tested in Murashige & Skoog medium, dicamba at 2.7 IxM was most effective in inducing and maintaining embryogenic cultures. Efficient plant regeneration was achieved
Plant regeneration via somatic embryogenesis in the seeded diploid bananaMusa ornataRoxb.
β Scribed by Sandra S. Cronauer-Mitra; A. D. Krikorian
- Publisher
- Springer
- Year
- 1988
- Tongue
- English
- Weight
- 853 KB
- Volume
- 7
- Category
- Article
- ISSN
- 0721-7714
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β¦ Synopsis
Somatic embryos of a seeded diploid ornamental banana (Musa ornata Roxb.) were obtained from zygotic embryos cultured on semi-solid Murashige and Skoog (MS) (1962) medium with the auxin 2,4-D (0.5, 1, 2 mg/l) and 5% CW. Removal of 2,4-D and transferral to Schenk and Hildebrandt (SH) (1972) salts with CW followed by basal MS led to embryo germination and growth. Plantlet production was obtained using filter paper bridges in liquid half-strength SH medium with 1% sucrose. The remarkable phenotypic fidelity of somatic embryos to that of zygotic embryos and the presence of a haustorium-like outgrowth on the somatic embryos is described.
π SIMILAR VOLUMES
We have established a high-frequency plant regeneration system via somatic embryogenesis from mature seeds of creeping bentgrass (Agrostis palustris Huds). The effects of 2,4-dichlorophenoxyacetic acid (2,4-D), 3.6-dichloroo-anisic acid (dicamba) and 6-benzyladenine (BA) on callus formation and embr