๐”– Bobbio Scriptorium
โœฆ   LIBER   โœฆ

Plant regeneration via somatic embryogenesis in creeping bentgrass (Agrostis palustrisHuds.)

โœ Scribed by Heng Zhong; C. Srinivasan; Mariam B. Sticklen

Publisher
Springer
Year
1991
Tongue
English
Weight
742 KB
Volume
10
Category
Article
ISSN
0721-7714

No coin nor oath required. For personal study only.

โœฆ Synopsis

We have established a high-frequency plant regeneration system via somatic embryogenesis from mature seeds of creeping bentgrass (Agrostis palustris Huds). The effects of 2,4-dichlorophenoxyacetic acid (2,4-D), 3.6-dichloroo-anisic acid (dicamba) and 6-benzyladenine (BA) on callus formation and embryogenesis were evaluated. Callus produced on the Murashige and Skoog (MS) (1962) medium containing 2,4-D had low embryogenic potency. In the presence of 30 ฮผM dicamba, addition of 2.25 to 9 ฮผM BA significantly enhanced embryogenic callus formation over dicamba alone. Optimum frequency of somatic embryogenesis was achieved on MS basal medium containing 30 ฮผM dicamba and 2.25 ฮผM BA. Over 80% of somatic embryos germinated and formed plantlets on half-strength MS basal medium. These plantlets grew normally in the greenhouse.

๐Ÿ“œ SIMILAR VOLUMES

Plant regeneration via somatic embryogen
Plant regeneration via somatic embryogenesis in ginger
โœ A. Kackar; S. R. Bhat; K. P. S. Chandel; S. K. Malik ๐Ÿ“‚ Article ๐Ÿ“… 1993 ๐Ÿ› Springer ๐ŸŒ English โš– 754 KB

Embryogenic callus cultures of ginger were induced from young leaf segments taken from in vitro shoot cultures. Among the four auxins tested in Murashige & Skoog medium, dicamba at 2.7 IxM was most effective in inducing and maintaining embryogenic cultures. Efficient plant regeneration was achieved

Plant regeneration via somatic embryogen
Plant regeneration via somatic embryogenesis in the seeded diploid bananaMusa ornataRoxb.
โœ Sandra S. Cronauer-Mitra; A. D. Krikorian ๐Ÿ“‚ Article ๐Ÿ“… 1988 ๐Ÿ› Springer ๐ŸŒ English โš– 853 KB

Somatic embryos of a seeded diploid ornamental banana (Musa ornata Roxb.) were obtained from zygotic embryos cultured on semi-solid Murashige and Skoog (MS) (1962) medium with the auxin 2,4-D (0.5, 1, 2 mg/l) and 5% CW. Removal of 2,4-D and transferral to Schenk and Hildebrandt (SH) (1972) salts wit