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Plant regeneration from rice anthers cryopreserved by an encapsulation/dehydration technique

✍ Scribed by María A. Marassi; Adriana Scocchi; Ana María Gonzalez


Book ID
111732688
Publisher
Society for In Vitro Biology
Year
2006
Tongue
English
Weight
347 KB
Volume
42
Category
Article
ISSN
1475-2689

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✍ Chun-Nong Huang; Jun-Hui Wang; Qiu-Sheng Yan; Xue-Qin Zhang; Qin-Feng Yan 📂 Article 📅 1995 🏛 Springer 🌐 English ⚖ 767 KB

Rice cells were precultured for 10 d in medium containing 60 g/L sucrose and subsequently for 1 d in medium supplemented with 0. 4 M sorbitol. After loading with 25%PVS2 at 22°C for 10 min and dehydration in 100%PVS2 at 0°C for 7. 5 min,they were plunged into liquid nitrogen directly. Survival was 4