Plant regeneration and genetic transformation ofLotus angustissimus

✍ Scribed by E. Nenz; F. Pupilli; F. Paolocci; F. Damiani; C. A. Cenci; S. Arcioni

• Publisher: Springer
• Year: 1996
• Tongue: English
• Weight: 951 KB
• Volume: 45
• Category: Article
• ISSN: 0167-6857
• DOI: 10.1007/bf00048758

✦ Synopsis

Culture conditions have been established for callus induction and growth from different explants in L. angustissimus L. Calli were obtained from hypocotyls, leaves, stems, cotyledons and roots cultured on media containing 2,4dichlorophenoxyacetic acid or c~-naphthaleneacetic acid with kinetin, N 6 -A2-isopentenyladenine or benzyladenine in different combinations and concentrations. Only those calli induced in presence of c~-naphthaleneacetic acid with benzyladenine or kinetin produced shoots. Calli induced from hypocotyl explants were the most efficient in regeneration of shoots. Transformation with an Agrobacterium rhizogenes binary vector carrying the plasmid pBI 121.1 is reported. The percentage of cotransformation was estimated by testing GUS activity in hairy roots. The integration of Ri T-DNA and the NPTII gene in transformed plants was confirmed by molecular analyses and in vitro culture of transgenic tissues in the presence of kanamycin.