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Effect of age and type of tissue on genetic transformation ofLotus corniculatusbyAgrobacterium tumefaciens

✍ Scribed by Ian P. Armstead; K. Judith Webb

Publisher
Springer
Year
1987
Tongue
English
Weight
294 KB
Volume
9
Category
Article
ISSN
0167-6857

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✦ Synopsis

Various experiments of Lotus corniculatus cv. Leo were infected with Agrobacterium tumefaciens strains C58 (wild-type) and GV3101 (control). A maximum of 83 per cent of cotyledons excised from 7 day old seedlings and 63 per cent of leaves excised from seedlings grown in vitro formed galls in culture. The genotype of the seedling had an effect on the response.

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