Front Matter ....Pages i-xi
An Overview of Protein Secretion in Yeast and Animal Cells (Yusong Guo, Feng Yang, Xiao Tang)....Pages 1-17
An Overview of Protein Secretion in Plant Cells (Kin Pan Chung, Yonglun Zeng)....Pages 19-32
Bioinformatics Analysis of Protein Secretion in Plants (Liyuan Chen)....Pages 33-43
Proteomic Analysis of Secreted Proteins from Cell Microenvironment (Subash Adhikari, Lan Chen, Peiwu Huang, Ruijun Tian)....Pages 45-58
Using Homology Modeling to Understand the Structural Basis of Specific Interaction of a Plant-Specific AtSar1a–AtSec23a Pair Involved in Protein ER Export (Yap-Shing Nim, Shuangli Sun, Kam-Bo Wong)....Pages 59-73
Analysis of Golgi-Mediated Protein Traffic in Plant Cells (Wenjin Shen, Zhidan Xiao, Jinbo Shen, Caiji Gao)....Pages 75-86
Analysis of Membrane Protein Topology in the Plant Secretory Pathway (Jinya Guo, Yansong Miao, Yi Cai)....Pages 87-95
Semiautomatic Segmentation of Plant Golgi Stacks in Electron Tomograms Using 3dmod (Keith Ka Ki Mai, Byung-Ho Kang)....Pages 97-104
3D Printing of Plant Golgi Stacks from Their Electron Tomographic Models (Keith Ka Ki Mai, Madison J. Kang, Byung-Ho Kang)....Pages 105-113
Transient Expression of Chimeric Fluorescent Reporter Proteins in Pollen Tubes to Study Protein Polar Secretion and Dynamics (Guitao Zhong, Ronghe Liu, Menglong Zhuang, Hao Wang)....Pages 115-124
Analysis of Actin-Based Intracellular Trafficking in Pollen Tubes (Yuxiang Jiang, Meng Zhang, Shanjin Huang)....Pages 125-136
Analysis of Phragmoplast Kinetics During Plant Cytokinesis (Pantelis Livanos, Mayank Chugh, Sabine Müller)....Pages 137-150
Immunofluorescence Analysis of Membrane-Associated Proteins for Clathrin-Mediated Endocytosis in Plant Root Cells (Chao Wang, Xu Yan, Tingting Meng, Tianwei Hu, Jianwei Pan)....Pages 151-157
In Vivo Interaction Studies by Measuring Förster Resonance Energy Transfer Through Fluorescence Lifetime Imaging Microscopy (FRET/FLIM) (Florian Fäßler, Peter Pimpl)....Pages 159-170
Analysis of Nanobody–Epitope Interactions in Living Cells via Quantitative Protein Transport Assays (Simone Früholz, Peter Pimpl)....Pages 171-182
A Secretion System for Cargo Protein Identification of Vacuolar Sorting Receptors (Jinbo Shen)....Pages 183-191
Identifying Novel Regulators of Vacuolar Trafficking by Combining Fluorescence Imaging-Based Forward Genetic Screening and In Vitro Pollen Germination (Qiang-Nan Feng, Yan Zhang)....Pages 193-198
Measuring Plant Protein Secretion (Emily R. Larson)....Pages 199-207
Transient Secretory Enzyme Expression in Leaf Protoplasts to Characterize SNARE Functional Classes in Conventional and Unconventional Secretion (Di Sansebastiano Gian Pietro, Barozzi Fabrizio)....Pages 209-221
The Organelle pH Estimate and Measurement in Plant Secretory Pathway (Jinbo Shen)....Pages 223-230
Analysis of Exocyst-Positive Organelle (EXPO)-Mediated Unconventional Protein Secretion (UPS) in Plant Cells (Yu Ding, Juan Wang)....Pages 231-241
Isolation of the Plant Exocyst Complex (King Pong Leung, Wilson Chun Yu Lau)....Pages 243-255
Using Microscopy Tools to Visualize Autophagosomal Structures in Plant Cells (Weili Lin, Xiaohong Zhuang)....Pages 257-266
Analysis of Plant Autophagy (Liang Chen, Faqiang Li, Shi Xiao)....Pages 267-280
Back Matter ....Pages 281-285