Plant Argonaute Proteins: Methods and Protocols

✍ Scribed by Alberto Carbonell (eds.)

Publisher: Humana Press
Year: 2017
Tongue: English
Leaves: 295
Series: Methods in Molecular Biology 1640
Edition: 1
Category: Library

No coin nor oath required. For personal study only.

✦ Synopsis

This volume provides the reader with step–by–step methods to study plant ARGONAUTE (AGO) functions. Chapters in Plant Argonaute Poteins: Methods and Protocols summarize the main biochemical, molecular and cytological methods to study AGO–small RNA complexes and their interactions with target RNAs, AGO subcellular localization, AGO association with polysomes, AGO role in meiosis and DNA repair, methods for the identification, cloning and characterization of AGO genes in different plant species, and non–radioactive protocols for small RNA detection. Finally, a series of chapters describing computational methods to study plant AGO function and evolution are provided. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Authoritative and practical, Plant Argonaute Proteins: Methods and Protocols aims to deliver the most complete and updated list of protocols to study plant AGO function.

✦ Table of Contents

Front Matter....Pages i-xi
Back Matter....Pages 1-21
....Pages 23-37

✦ Subjects

Plant Sciences

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