Plant Programmed Cell Death: Methods and Protocols

✍ Scribed by Laura De Gara,Vittoria Locato (eds.)

• Publisher: Humana Press
• Year: 2018
• Tongue: English
• Leaves: 186
• Series: Methods in Molecular Biology 1743
• Edition: 1
• Category: Library

✦ Synopsis

This detailed volume explores numerous protocols that can be specifically used for studying plant programmed cell death (PCD), a mechanism involved in a number of physiological and pathological processes that are triggered by developmental requirements as well as changing/adverse environmental conditions. Rich in methodological recommendations, this book enables readers to perform the described analyses, alerting them to possible difficulties and showing how the methods can also be applied in different contexts and/or on different plant matrices. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Authoritative and practical, Plant Programmed Cell Death: Methods and Protocols provides essential methodological information that will help researchers to expand the field of plant PCD studies.

✦ Table of Contents

Front Matter ....Pages i-x
Programmed Cell Death in Plants: An Overview (Vittoria Locato, Laura De Gara)....Pages 1-8
Investigation of Morphological Features of Autophagy During Plant Programmed Cell Death (Alessio Papini)....Pages 9-19
Markers of Developmentally Regulated Programmed Cell Death and Their Analysis in Cereal Seeds (Fernando Domínguez, Francisco Javier Cejudo)....Pages 21-37
Measurement of Hypersensitive Cell Death Triggered by Avirulent Bacterial Pathogens in Arabidopsis (Zahra Imanifard, Elodie Vandelle, Diana Bellin)....Pages 39-50
Immunity-Associated Programmed Cell Death as a Tool for the Identification of Genes Essential for Plant Innate Immunity (Bangjun Zhou, Lirong Zeng)....Pages 51-63
Analysis of Mitochondrial Markers of Programmed Cell Death (Dong Xiao, Huyi He, Wenjing Huang, Thet Lwin Oo, Aiqin Wang, Long-Fei He)....Pages 65-71
Studying Retrograde Signaling in Plants (Martyna Broda, Olivier Van Aken)....Pages 73-85
ROS and Cell Death in Tomato Roots Infected by Meloidogyne Incognita (Paola Leonetti)....Pages 87-95
Detection of Reactive Oxygen and Nitrogen Species (ROS/RNS) During Hypersensitive Cell Death (Laura C. Terrón-Camero, Eliana Molina-Moya, María Sanz-Fernández, Luisa M. Sandalio, María C. Romero-Puertas)....Pages 97-105
DNA Diffusion Assay Applied to Plant Cells (Anca Macovei, Mattia Donà, Daniela Carbonera, Alma Balestrazzi)....Pages 107-115
Analysis of Reactive Carbonyl Species Generated Under Oxidative Stress (Jun’ichi Mano, Md. Sanaullah Biswas)....Pages 117-124
In Vivo Analysis of Calcium Levels and Glutathione Redox Status in Arabidopsis Epidermal Leaf Cells Infected with the Hypersensitive Response-Inducing Bacteria Pseudomonas syringae pv. tomato AvrB (PstAvrB) (Fabrizio Gandolfo Doccula, Laura Luoni, Smrutisanjita Behera, Maria Cristina Bonza, Alex Costa)....Pages 125-141
Measurement of Cyclic GMP During Plant Hypersensitive Disease Resistance Response (Jian Chen, Diana Bellin, Elodie Vandelle)....Pages 143-151
Detection of MAPK3/6 Phosphorylation During Hypersensitive Response (HR)-Associated Programmed Cell Death in Plants (Qingyu Wu, David Jackson)....Pages 153-161
Measurement of the Caspase-1-Like Activity of Vacuolar Processing Enzyme in Plants (Noriyuki Hatsugai, Ikuko Hara-Nishimura)....Pages 163-171
Plant Cell Cultures as Model Systems to Study Programmed Cell Death (Sara Cimini, Maria Beatrice Ronci, Elisabetta Barizza, Maria Concetta de Pinto, Vittoria Locato, Fiorella Lo Schiavo et al.)....Pages 173-186
Back Matter ....Pages 187-188

✦ Subjects

Plant Sciences