Plant Cell Morphogenesis: Methods and Protocols

✍ Scribed by Fatima Cvrčková, Viktor Žárský

• Publisher: Springer New York; Humana
• Year: 2019
• Tongue: English
• Leaves: 379
• Series: Methods in Molecular Biology 1992
• Edition: 2nd ed.
• Category: Library

✦ Synopsis

This book collects techniques to continue exploring post-genomic land plant biology though the wisdom and skills accumulated from work on the founding molecular biology models that can now guide research into other species, including crop plants. Beginning with the visualization of plant cell structures, the volume moves on to cover digital image analysis protocols, qualitative and quantitative detection of the organization and dynamics of individual intracellular structures, the manipulation of intracellular structures, as well as techniques for studying model cell types. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Authoritative and fully updated, Plant Cell Morphogenesis: Methods and Protocols, Second Edition serves as an ideal source of inspiration for further research into the morphogenesis of plant cells, tissues, and organs.

✦ Table of Contents

Front Matter ....Pages i-xi
Essential Methods of Plant Sample Preparation for Light Microscopy (Aleš Soukup, Edita Tylová)....Pages 1-26
Selected Simple Methods of Plant Cell Wall Histochemistry and Staining for Light Microscopy (Aleš Soukup)....Pages 27-42
Chemical Fixation, Immunofluorescence, and Immunogold Labeling of Electron Microscopical Sections (Ilse Foissner, Margit Hoeftberger)....Pages 43-62
Essential Methods of Plant Sample Preparation for High-Resolution Scanning Electron Microscopy at Room Temperature (Jana Nebesářová)....Pages 63-76
Fluorescence Lifetime Imaging of Plant Cell Walls (Christine Terryn, Gabriel Paës)....Pages 77-82
Raman Spectroscopy in Nonwoody Plants (Dorota Borowska-Wykręt, Mateusz Dulski)....Pages 83-107
Image Analysis: Basic Procedures for Description of Plant Structures (Jana Albrechtová, Zuzana Kubínová, Aleš Soukup, Jiří Janáček)....Pages 109-119
From Data to Illustrations: Common (Free) Tools for Proper Image Data Handling and Processing (Fatima Cvrčková)....Pages 121-133
Visualizing and Quantifying In Vivo Cortical Cytoskeleton Structure and Dynamics (Amparo Rosero, Denisa Oulehlová, Viktor Žárský, Fatima Cvrčková)....Pages 135-149
Quantitative and Comparative Analysis of Global Patterns of (Microtubule) Cytoskeleton Organization with CytoskeletonAnalyzer2D (Birgit Möller, Luise Zergiebel, Katharina Bürstenbinder)....Pages 151-171
Using FM Dyes to Study Endomembranes and Their Dynamics in Plants and Cell Suspensions (Adriana Jelínková, Kateřina Malínská, Jan Petrášek)....Pages 173-187
Transient Gene Expression as a Tool to Monitor and Manipulate the Levels of Acidic Phospholipids in Plant Cells (Lise C. Noack, Přemysl Pejchar, Juraj Sekereš, Yvon Jaillais, Martin Potocký)....Pages 189-199
The Photoconvertible Fluorescent Protein Dendra2 Tag as a Tool to Investigate Intracellular Protein Dynamics (Alexandra Lešková, Zuzana Kusá, Mária Labajová, Miroslav Krausko, Ján Jásik)....Pages 201-214
Cellular Force Microscopy to Measure Mechanical Forces in Plant Cells (Mateusz Majda, Aleksandra Sapala, Anne-Lise Routier-Kierzkowska, Richard S. Smith)....Pages 215-230
Optical Trapping in Plant Cells (Tijs Ketelaar, Norbert de Ruijter, Stefan Niehren)....Pages 231-238
Sequential Replicas: Method for In Vivo Imaging of Plant Organ Surfaces that Undergo Deformation (Dorota Kwiatkowska, Sandra Natonik-Białoń, Agata Burian)....Pages 239-255
Time-Lapse Imaging of Developing Shoot Meristems Using A Confocal Laser Scanning Microscope (Olivier Hamant, Pradeep Das, Agata Burian)....Pages 257-268
Quantifying Plant Growth and Cell Proliferation with MorphoGraphX (Soeren Strauss, Aleksandra Sapala, Daniel Kierzkowski, Richard S. Smith)....Pages 269-290
Kinematic Characterization of Root Growth by Means of Stripflow (Tobias I. Baskin, Ellen Zelinsky)....Pages 291-305
Automated Image Acquisition and Morphological Analysis of Cell Growth Mutants in Physcomitrella patens (Giulia Galotto, Jeffrey P. Bibeau, Luis Vidali)....Pages 307-322
Live Cell Imaging of Arabidopsis Root Hairs (Tijs Ketelaar)....Pages 323-327
Morphological Analysis of Leaf Epidermis Pavement Cells with PaCeQuant (Birgit Möller, Yvonne Poeschl, Sandra Klemm, Katharina Bürstenbinder)....Pages 329-349
Imaging of Developing Metaxylem Vessel Elements in Cultured Hypocotyls (Takema Sasaki, Yoshihisa Oda)....Pages 351-358
Antisense Oligodeoxynucleotide-Mediated Gene Knockdown in Pollen Tubes (Martin Potocký, Radek Bezvoda, Přemysl Pejchar)....Pages 359-365
Plant Cell Lines in Cell Morphogenesis Research: From Phenotyping to -Omics (Petr Klíma, Vojtěch Čermák, Miroslav Srba, Karel Müller, Jan Petrášek, Josef Šonka et al.)....Pages 367-376
Back Matter ....Pages 377-380

✦ Subjects

Life Sciences; Plant Physiology; Plant Sciences