"This book offers 19 detailed protocols on the use of induced mutations in crop breeding and functional genomics studies, which cover topics including chemical and physical mutagenesis, phenotypic screening methods, traditional TILLING and TILLING by sequencing, doubled haploidy, targeted genome edi
Plant mutation breeding and biotechnology
✍ Scribed by Q Y Shu
- Publisher
- CABI
- Year
- 2012
- Tongue
- English
- Leaves
- 613
- Category
- Library
No coin nor oath required. For personal study only.
✦ Table of Contents
TABLE OF CONTENTS......Page 10
FOREWORD......Page 6
PREFACE......Page 8
SECTION I – Concepts, Historical Development and Genetic Basis......Page 12
C01 Plant Mutagenesis in Crop Improvement: Basic Terms and Applications......Page 14
C02 A Brief History of Plant Mutagenesis......Page 26
C03 The Structure and Regulation of Genes and Consequences of Genetic Mutations......Page 36
C04 Mutation Categories......Page 52
C05 DNA Repair Pathways and Genes in Plant......Page 62
C06 Double-Stranded DNA Break, Repair and Associated Mutations......Page 76
SECTION II – Mutagens and Induced Mutagenesis......Page 86
C07 Mutagenic Radiations: X-Rays, Ionizing Particles and Ultraviolet......Page 88
C08 Gamma Irradiation......Page 96
C09 Ion Beam Radiation Mutagenesis......Page 104
C10 Ion Implantation Mutagenesis......Page 112
C11 Effects of Radiation on Living Cells and Plants......Page 128
C12 Chemical Mutagenesis......Page 140
C13 Sodium Azide as a Mutagen......Page 164
SECTION III – Mutation Induction and Mutant Development......Page 172
C14 Methodology for Physical and Chemical Mutagenic Treatments......Page 174
C15 Chimeras and Mutant Gene Transmission......Page 186
C16 Chimeras: Properties and Dissociation in Vegetatively Propagated Plants......Page 196
C17 Mutation Induction in Cytoplasmic Genomes......Page 208
C18 Strategies and Approaches in Mutant Population Development for Mutant Selection in Seed Propagated Crops......Page 214
C19 Irradiation – Facilitated Chromosomal Translocation: Wheat as an Example......Page 228
C20 Molecular Techniques and Methods for Mutation Detection and Screening in Plants......Page 246
C21 Discovery of Chemically Induced Mutations by TILLING......Page 262
C22 A Protocol for TILLING and Eco-TILLING......Page 274
C23 Applications of DNA Marker Techniques in Plant Mutation Research......Page 292
SECTION IV – Mutation Breeding......Page 304
C24 Principles and Applications of Plant Mutation Breeding......Page 306
C25 Mutant Phenotyping and Pre-Breeding in Barley......Page 332
C26 Mutation Breeding of Vegetatively Propagated Crops......Page 352
C27 Uses of TILLING For Crop Improvement......Page 364
C28 Applications of In Vitro Techniques in Mutation Breeding of Vegetatively Propagated Crops......Page 376
C29 Haploid Mutagenesis......Page 392
C30 Use of Irradiated Pollen to Induce Parthenogenesis and Haploid Production in Fruit Crops......Page 416
C31 Herbicide-Tolerant Crops Developed from Mutations......Page 428
C32 Mutation Breeding for Fatty Acid Composition in Soybean......Page 442
C33 Genetic Improvement of Basmati Rice Through Mutation Breeding......Page 450
C34 Mutation Breeding of Sweet Cherry (Prunus avium L.) var. 0900 Ziraat......Page 458
SECTION V – Mutations in Functional Genomics......Page 466
C35 Cloning Genes for Mineral Uptake: Examples Using Rice Mutants......Page 468
C36 Molecular Genetics of Symbiotic Plant Microbe Interactions in a Model Legume, Lotus Japonicus......Page 476
C37 Mutational Dissection of the Phytochrome Genetic Systems in Rice......Page 484
C38 T-DNA Insertion Mutagenesis......Page 494
C39 Transposon Mutagenesis for Functional Genomics......Page 512
C40 Site-Directed Mutagenesis in Higher Plants......Page 528
C41 Phenomics in Plant Biological Research and Mutation Breeding......Page 540
APPENDIX......Page 566
A......Page 596
C......Page 597
D......Page 598
E......Page 599
G......Page 600
I......Page 602
K......Page 603
M......Page 604
P......Page 606
S......Page 609
T......Page 611
V......Page 612
Z......Page 613
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