Biotechnologies for Plant Mutation Breeding: Protocols

✍ Scribed by Joanna Jankowicz-Cieslak, Thomas H. Tai, Jochen Kumlehn, Bradley J. Till (eds.)

• Publisher: Springer International Publishing
• Year: 2017
• Tongue: English
• Leaves: 343
• Edition: 1
• Category: Library

✦ Synopsis

This book is open access under a CC BY-NC 2.5 license.

This book offers 19 detailed protocols on the use of induced mutations in crop breeding and functional genomics studies, which cover topics including chemical and physical mutagenesis, phenotypic screening methods, traditional TILLING and TILLING by sequencing, doubled haploidy, targeted genome editing, and low-cost methods for the molecular characterization of mutant plants that are suitable for laboratories in developing countries. The collection of protocols equips users with the techniques they need in order to start a program on mutation breeding or functional genomics using both forward and reverse-genetic approaches. Methods are provided for seed and vegetatively propagated crops (e.g. banana, barley, cassava, jatropha, rice) and can be adapted for use in other species.

✦ Table of Contents

Front Matter....Pages i-xx
Front Matter....Pages 1-1
Mutagenesis for Crop Breeding and Functional Genomics....Pages 3-18
Front Matter....Pages 19-19
Chemical and Physical Mutagenesis in Jatropha curcas ....Pages 21-38
Chemical Mutagenesis and Chimera Dissolution in Vegetatively Propagated Banana....Pages 39-54
Mutation Induction Using Gamma Irradiation and Embryogenic Cell Suspensions in Plantain (Musa spp.)....Pages 55-71
Optimisation of Somatic Embryogenesis in Cassava....Pages 73-89
Creation of a TILLING Population in Barley After Chemical Mutagenesis with Sodium Azide and MNU....Pages 91-111
Site-Directed Mutagenesis in Barley by Expression of TALE Nuclease in Embryogenic Pollen....Pages 113-128
Doubled Haploidy as a Tool for Chimaera Dissolution of TALEN-Induced Mutations in Barley....Pages 129-141
Front Matter....Pages 143-143
Field Evaluation of Mutagenized Rice Material....Pages 145-156
Root Phenotyping Pipeline for Cereal Plants....Pages 157-172
Breeding New Aromatic Rice with High Iron Using Gamma Radiation and Hybridization....Pages 173-191
Utilising NIRS for Qualitative and Non-destructive Identification of Seed Mutants in Large Populations....Pages 193-202
Proteome Analyses of Jatropha curcas ....Pages 203-223
Front Matter....Pages 225-225
Low-Cost Methods for DNA Extraction and Quantification....Pages 227-239
A Protocol for Benchtop Extraction of Single-Strand-Specific Nucleases for Mutation Discovery....Pages 241-251
A Protocol for Validation of Doubled Haploid Plants by Enzymatic Mismatch Cleavage....Pages 253-262
Bioinformatics-Based Assessment of the Relevance of Candidate Genes for Mutation Discovery....Pages 263-280
Mutation Detection by Analysis of DNA Heteroduplexes in TILLING Populations of Diploid Species....Pages 281-303
Determining Mutation Density Using Restriction Enzyme Sequence Comparative Analysis (RESCAN)....Pages 305-321
Next-Generation Sequencing for Targeted Discovery of Rare Mutations in Rice....Pages 323-340

✦ Subjects

Plant Breeding/Biotechnology;Agriculture;Genetic Engineering;Plant Genetics & Genomics