## Abstract A single C/T difference between gene and cDNA within the 5β²βuntranslated region of the wheat mtDNAβencoded __cox2__ (cytochrome oxidase subunit 2) sequence catalyzed the discovery of CβtoβU RNA editing in plant mitochondria by a group at Dalhousie University in Halifax, Nova Scotia [Cov
Plant mitochondrial RNA editing: The Strasbourg chapter
β Scribed by Jean Michel Grienenberger
- Publisher
- John Wiley and Sons
- Year
- 2009
- Tongue
- English
- Weight
- 70 KB
- Volume
- 61
- Category
- Article
- ISSN
- 1521-6543
- DOI
- 10.1002/iub.277
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β¦ Synopsis
Abstract
For 8 years, it was not understood why certain genes of plant mitochondria contain CGG (arginine) codons at positions where tryptophan codons (UGG) are present in the corresponding genes of nonplant species. Identification and sequencing of a tRNA^Trp^ gene showed that it is not able to decode the CGG codon. Analysis of different discrepancies in the sequences of plant mitochondrial proteins prompted us to determine directly the corresponding RNA sequences. These experiments showed that plant mitochondrial transcripts are subject to RNA editing that changes C into U, resulting in a better phylogenetic conservation of protein sequences [Gualberto et al. (1989) Nature 341, 660β662]. Β© 2009 IUBMB IUBMB Life, 61: 1110β1113, 2009
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The barley (Hordeum vulgare) FO-ATP synthase subunit 9 (atp9) was isolated from the mitochondrial DNA. Two copies of atp9 are present in barley mitochondria and 2 major transcripts of 2.7 kb and 1.2 kb were detected on RNA blots. RNA editing as C-to-U conversion occurs in barley atp9 in seven positi