Plant Innate Immunity: Methods and Protocols

✍ Scribed by Walter Gassmann

• Publisher: Springer New York; Humana
• Year: 2019
• Tongue: English
• Leaves: 266
• Series: Methods in Molecular Biology 1991
• Edition: 1st ed.
• Category: Library

✦ Synopsis

The volume presents valuable methods that look at important biological processes not traditionally assayed in the study of plant immunity, and at non-model systems. The chapters in this book cover topics such as identifying host targets of acetylating effectors by immunoprecipitation; quantifying ATP release from plant cells; protein-DNA interactions; DNA methylation; measurement and playback of leaf vibrations; natural infection routes of Xanthomonas campestris pv. campestris using Arabidopsis; and isolating favorable plant-growth-promoting bacteria from the phytosphere. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Cutting-edge and thorough, Plant Innate Immunity: Methods and Protocols is an essential resource for all researchers interested in expanding their knowledge and learning new techniques in this ever-growing field.

✦ Table of Contents

Front Matter ....Pages i-xiii
Using Bioinformatics and Molecular Biology to Streamline Construction of Effector Libraries for Phytopathogenic Pseudomonas syringae Strains (Jay Jayaraman, Morgan K. Halane, Sera Choi, Honour C. McCann, Kee Hoon Sohn)....Pages 1-12
Immunoprecipitation Under Non-Denaturing or Denaturing Conditions of Lysine-Acetylated Proteins Expressed in Planta (Maxime Escouboué, Laurent Deslandes)....Pages 13-21
Protein Acetylation in Pathogen Virulence and Host Defense: In Vitro Detection of Protein Acetylation by Radiolabeled Acetyl Coenzyme A (Karl J. Schreiber, Jennifer D. Lewis)....Pages 23-32
A Novel Assay Based on Confocal Microscopy to Test for Pathogen Silencing Suppressor Functions (Mustafa Adhab, James E. Schoelz)....Pages 33-42
Quantification of Extracellular ATP in Plant Suspension Cell Cultures (Sowmya R. Ramachandran, Sonika Kumar, Kiwamu Tanaka)....Pages 43-54
Measuring Pectin Properties to Track Cell Wall Alterations During Plant–Pathogen Interactions (Gerit Bethke, Jane Glazebrook)....Pages 55-60
Method to Study Dynamics of Membrane-Bound Plant Transcription Factors During Biotic Interactions in Tomato (Supriyo Chowdhury, Payel Bhattacharjee, Shrabani Basak, Shreya Chowdhury, Pallob Kundu)....Pages 61-68
Preparation of Plant Material for Analysis of Protein–Nucleic Acid Interactions by FRET-FLIM (Maxime Escouboué, Laurent Camborde, Alain Jauneau, Elodie Gaulin, Laurent Deslandes)....Pages 69-77
Analysis of DNA Methylation Profile in Plants by Chop-PCR (Pratiti Dasgupta, Shubho Chaudhuri)....Pages 79-90
Change in Nucleosome Dynamics During Stress Responses in Plants (Amit Paul, Shubho Chaudhuri)....Pages 91-100
Measuring Cell Ploidy Level in Arabidopsis thaliana by Flow Cytometry (Leiyun Yang, Zhixue Wang, Jian Hua)....Pages 101-106
A Method for Investigating the Pseudomonas syringae-Arabidopsis thaliana Pathosystem Under Various Light Environments (Daniel L. Leuchtman, Anthony D. Shumate, Walter Gassmann, Emmanuel Liscum)....Pages 107-113
Isolation and Characterization of Plant Metabolite Signals that Induce Type III Secretion by the Plant Pathogen Pseudomonas syringae (Conner J. Rogan, Jeffrey C. Anderson)....Pages 115-126
Quantification of Cauline Leaf Abscission in Response to Plant Pathogens (O. Rahul Patharkar)....Pages 127-139
Methods for Replicating Leaf Vibrations Induced by Insect Herbivores (Sabrina C. J. Michael, Heidi A. Appel, Reginald B. Cocroft)....Pages 141-157
Infection Assay for Xanthomonas campestris pv. campestris in Arabidopsis thaliana Mimicking Natural Entry via Hydathodes (Marieke van Hulten, Sayantani Chatterjee, Harrold A. van den Burg)....Pages 159-185
The Apple Fruitlet Model System for Fire Blight Disease (Sara M. Klee, Judith P. Sinn, Timothy W. McNellis)....Pages 187-198
Generating Transgenic Arabidopsis Plants for Functional Analysis of Pathogen Effectors and Corresponding R Proteins (Sharon Pike, Walter Gassmann, Jianbin Su)....Pages 199-206
Identification of Novel Pararetroviral Promoters for Designing Efficient Plant Gene Expression Systems (Ankita Shrestha, Ahamed Khan, Nrisingha Dey)....Pages 207-222
Biochemical and Molecular Characterization of Novel Pararetroviral Promoters in Plants (Ahamed Khan, Ankita Shrestha, Nrisingha Dey)....Pages 223-236
Sampling and Handling of Soil to Identify Microorganisms with Impacts on Plant Growth (Robert J. Kremer)....Pages 237-246
A New Strategy for the Selection of Epiphytic and Endophytic Bacteria for Enhanced Plant Performance (Eduardo Balsanelli, Vânia Carla Pankievicz, Valter Antonio Baura, Fábio de Oliveira Pedrosa, Emanuel Maltempi de Souza)....Pages 247-256
Back Matter ....Pages 257-260

✦ Subjects

Life Sciences; Plant Sciences