Innate Immune Activation: Methods and Protocols

✍ Scribed by Dominic De Nardo,Christine M De Nardo (eds.)

Publisher: Humana Press
Year: 2018
Tongue: English
Leaves: 255
Series: Methods in Molecular Biology 1714
Edition: 1
Category: Library

No coin nor oath required. For personal study only.

✦ Synopsis

This volume presents standard approaches and the most recent technical advances used to study innate immune activation. Chapters detail the assessment of macrophage activation, measuring innate immune responses to bacterial viability, quantification of secreted proteins, reporter systems, protocols examining specific innate immune activation by TLRs, RLRs, cGAS, and inflammasomes. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Authoritative and practical, Innate Immune Activation: Methods and Protocols aims to be a useful and informative reference tool for further study into this vital field.

✦ Table of Contents

Front Matter ....Pages i-xiii
Emerging Concepts in Innate Immunity (Karin Pelka, Dominic De Nardo)....Pages 1-18
Bioinformatic Assessment of Macrophage Activation by the Innate Immune System (Thomas Ulas, Joachim L. Schultze, Marc Beyer)....Pages 19-40
Generation of Genetic Knockouts in Myeloid Cell Lines Using a Lentiviral CRISPR/Cas9 System (Paul J. Baker, Seth L. Masters)....Pages 41-55
Modeling Primary Human Monocytes with the Trans–Differentiation Cell Line BLaER1 (Moritz M. Gaidt, Francesca Rapino, Thomas Graf, Veit Hornung)....Pages 57-66
Measurement of NF-κB Activation in TLR-Activated Macrophages (Orna Ernst, Sharat J. Vayttaden, Iain D. C. Fraser)....Pages 67-78
Biochemical Isolation of the Myddosome from Murine Macrophages (Yunhao Tan, Jonathan C. Kagan)....Pages 79-95
Generation of Innate Immune Reporter Cells Using Retroviral Transduction (Yamel Cardona Gloria, Eicke Latz, Dominic De Nardo)....Pages 97-117
Examining Myddosome Formation by Luminescence-Based Mammalian Interactome Mapping (LUMIER) (Olaf-Oliver Wolz, Manfred Koegl, Alexander N. R. Weber)....Pages 119-130
Inflammatory Caspases: Activation and Cleavage of Gasdermin-D In Vitro and During Pyroptosis (Yue Zhao, Jianjin Shi, Feng Shao)....Pages 131-148
Detection of ASC Speck Formation by Flow Cytometry and Chemical Cross-linking (Florian Hoss, Verena Rolfes, Mariana R. Davanso, Tarcio T. Braga, Bernardo S. Franklin)....Pages 149-165
Measuring Innate Immune Responses to Bacterial Viability (Julien Moretti, Nicolas Vabret, J. Magarian Blander)....Pages 167-190
Methods to Study Cell Swelling-Induced Inflammasome Activation (Vincent Compan, Pablo Pelegrín)....Pages 191-197
Detecting Release of Bacterial dsDNA into the Host Cytosol Using Fluorescence Microscopy (Roland Felix Dreier, José Carlos Santos, Petr Broz)....Pages 199-213
Quantitative Proteomics of Secreted Proteins (Annika Frauenstein, Felix Meissner)....Pages 215-227
Simultaneous Detection of Cellular Viability and Interleukin-1β Secretion from Single Cells by ELISpot (Stephanie A. Conos, Lisa M. Lindqvist, James E. Vince)....Pages 229-236
Detection and Quantification of MAVS Aggregation via Confocal Microscopy (Tan A. Nguyen, Lachlan Whitehead, Ken C. Pang)....Pages 237-247
Back Matter ....Pages 249-251

✦ Subjects

Immunology

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