Phytochrome — all regions marked by a set of monoclonal antibodies reflect conformational changes

✍ Scribed by Hansjörg A. W. Schneider-Poetsch; Birgit Braun; Wolfhart Rüdiger

• Book ID: 104753156
• Publisher: Springer-Verlag
• Year: 1989
• Tongue: English
• Weight: 421 KB
• Volume: 177
• Category: Article
• ISSN: 0032-0935
• DOI: 10.1007/bf00392619

✦ Synopsis

Monoclonal antibodies to defined locations on six regions of the phytochrome molecule (from Arena sativa L. or Zea mays L.) were each found to have a different affinity toward the farred-absorbing form of phytochrome (Pfr) and the red-absorbing form (Pr). The differences were small, but were consistently shown by antibodies which bind to the vicinity of the aminoterminus, the carboxylterminus and to sequences in between. It seems that the conformational differences between Pr and Pfr extend over the whole molecule in as far as it is represented by these regions and the antibodies binding to them.