𝔖 Bobbio Scriptorium
✦   LIBER   ✦

Physiology and pathophysiology of poly(ADP-ribosyl)ation

✍ Scribed by Alexander Bürkle

Publisher
John Wiley and Sons
Year
2001
Tongue
English
Weight
247 KB
Volume
23
Category
Article
ISSN
0265-9247

No coin nor oath required. For personal study only.

✦ Synopsis

One of the immediate eukaryotic cellular responses to DNA breakage is the covalent post-translational modification of nuclear proteins with poly(ADP-ribose) from NAD+ as precursor, mostly catalysed by poly(ADP-ribose) polymerase-1 (PARP-1). Recently several other polypeptides have been shown to catalyse poly(ADP-ribose) formation. Poly(ADP-ribosyl)ation is involved in a variety of physiological and pathophysiological phenomena. Physiological functions include its participation in DNA-base excision repair, DNA-damage signalling, regulation of genomic stability, and regulation of transcription and proteasomal function, supporting the previously observed correlation of cellular poly(ADP-ribosyl)ation capacity with mammalian life. The pathophysiology effects are mediated through PARP-1 overactivity, which can cause cell suicide by NAD+ depletion. It is apparent that the latter effect underlies the pathogenesis of a wide range of disease states including type-1 diabetes, ischaemic infarcts in various organs, and septic or haemorrhagic shock. Therefore pharmacological modulation of poly(ADP-ribosyl)ation may prove to be an exciting option for various highly prevalent, disabling and even lethal diseases.

📜 SIMILAR VOLUMES

Poly(ADP-ribosyl)ation inhibitors: Promi
Poly(ADP-ribosyl)ation inhibitors: Promising drug candidates for a wide variety of pathophysiologic conditions
✍ Sascha Beneke; Joerg Diefenbach; Alexander Bürkle 📂 Article 📅 2004 🏛 John Wiley and Sons 🌐 French ⚖ 116 KB 👁 1 views

## Abstract Poly(ADP‐ribose) polymerases are involved in many aspects of regulation of cellular functions. Using NAD^+^ as a substrate, they catalyse the covalent transfer of ADP‐ribose units onto several acceptor proteins to form a branched ADP‐ribose polymer. The best characterised and first disc

How to kill tumor cells with inhibitors
How to kill tumor cells with inhibitors of poly(ADP-ribosyl)ation
✍ Aswin Mangerich; Alexander Bürkle 📂 Article 📅 2010 🏛 John Wiley and Sons 🌐 French ⚖ 455 KB 👁 1 views

## Abstract Poly(ADP‐ribosyl)ation is a post‐translational modification catalyzed by the enzyme family of poly(ADP‐ribose) polymerases (PARPs). PARPs exhibit pleiotropic cellular functions ranging from maintenance of genomic stability and chromatin remodeling to regulation of cell death, thereby re

Very low concentrations of arsenite supp
Very low concentrations of arsenite suppress poly(ADP-ribosyl)ation in mammalian cells
✍ Andrea Hartwig; Anke Pelzer; Monika Asmuss; Alexander Bürkle 📂 Article 📅 2002 🏛 John Wiley and Sons 🌐 French ⚖ 211 KB 👁 1 views

## Abstract Arsenite is a naturally occurring environmental pollutant of major concern, since adverse health effects including cancer of skin and internal organs have been attributed to chronic arsenic exposure especially via drinking water. Arsenite is not a significant inducer of point mutations

A cellular survival switch: poly(ADP-rib
A cellular survival switch: poly(ADP-ribosyl)ation stimulates DNA repair and silences transcription
✍ Mathias Ziegler; Shiao Li Oei 📂 Article 📅 2001 🏛 John Wiley and Sons 🌐 English ⚖ 139 KB 👁 1 views

## Abstract Poly(ADP‐ribosyl)ation is a post‐translational modification occurring in the nucleus. The most abundant and best‐characterized enzyme catalyzing this reaction, poly(ADP‐ribose) polymerase 1 (PARP1), participates in fundamental nuclear events. The enzyme functions as molecular “nick sens

Quantitative Nonisotopic Immuno-Dot-Blot
Quantitative Nonisotopic Immuno-Dot-Blot Method for the Assessment of Cellular Poly(ADP-ribosyl)ation Capacity
✍ Ragen Pfeiffer; Christine Brabeck; Alexander Bürkle 📂 Article 📅 1999 🏛 Elsevier Science 🌐 English ⚖ 95 KB

did not appear to migrate out of the gel, causing band aggregation toward the bottom. We also used the Spreadex gels in conventional gel tanks such as the BRL Horizon 11.14. without buffer recirculation or temperature control; results were much inferior to those obtained with the instrument used he