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Physiological characterization of a plastidic signal required for nitrate-induced appearance of nitrate and nitrite reductases

✍ Scribed by R. Oelmüller; C. Schuster; H. Mohr

Book ID
104754583
Publisher
Springer-Verlag
Year
1988
Tongue
English
Weight
738 KB
Volume
174
Category
Article
ISSN
0032-0935

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✦ Synopsis

We compared the response of NO~-induced nitrate-reductase (NR) and nitrite-reductase (NIR) levels in virtually carotenoid-free far-redlight-grown mustard (Sinapis alba L.) cotyledons following a photooxidative treatment of the plastids. The cytosolic localization of NR and the plastidic localization of NIR were confirmed with this approach. Emphasis was on a plastidic factor previously postulated to be involved obligatorily in the transcriptional control of nuclear genes coding for proteins destined for the chloroplast. Photooxidative damage of the plastid would be to destroy the ability of the organelle to send off this signal. Dependency of NIR and NR induction by NO3on the plastidic factor is described in detail, and it is concluded that requirement for the plastidic factor is relatively high in the case of NR while factor requirement to allow induction is low in the case of NIR. The data indicate that in the case of NIR the photooxidative damage done to the plastid also affects accumulation of the enzyme directly. Since this effect is absent in the case of cytosolic NR, induction of NR is a particularly suitable system for further molecular studies of the plastidic factor and its mode of action.

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which is cytosolic, as if it were a plastidic protein. The appearance of NR3 depends on the plastidic factor in principally the same way as that of NR~ and NR2 whereas NR 4 is totally independent of the plastidic factor. The data document particular kinds of interaction between controlling factors