During transformation of mustard seedlings cotyledons from storage organs to photosynthetically competent leaves, a process which occurs during the first 4 d after sowing, total glutamine-synthetase (GS, EC 6.3.1.2) activity increases from zero to the high level usually observed in green leaves. In
Effect of nitrate, ammonium, light and a plastidic factor on the appearance of multiple forms of nitrate reductase in mustard (Sinapis albaL.) cotyledons
β Scribed by C. Schuster; S. Schmidt; H. Mohr
- Publisher
- Springer-Verlag
- Year
- 1989
- Tongue
- English
- Weight
- 915 KB
- Volume
- 177
- Category
- Article
- ISSN
- 0032-0935
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β¦ Synopsis
which is cytosolic, as if it were a plastidic protein.
The appearance of NR3 depends on the plastidic factor in principally the same way as that of NR~ and NR2 whereas NR 4 is totally independent of the plastidic factor. The data document particular kinds of interaction between controlling factors (light, nitrate, ammonium, plastidic factor) which affect gene expression in plants. These intricacies of regulation have so far not been considered in molecular studies on NR-gene expression.
π SIMILAR VOLUMES
Nitrite reductase (NIR; EC 1.7.7.1) is a central enzyme in nitrate assimilation and is localized in plastids. The present study concerns the regulation of the appearance of NIR in cotyledons of the mustard (Sinapis alba L.) seedling. It was shown that light exerts its positive control over the nitra
An increase of glutathione reductase (GR; EC 1.6.4.2) activity during the transformation of mustard (Sinapis alba L.) cotyledons from storage organs to photosynthetically competent leaves was previously found to be controlled by light acting via phytochrome (Drumm, H., Mohr, H., Z. Naturforsch. 28c
Nitrate-induced and phytochrome-modulated appearance of nitrate reductase (NR; EC 1.6.6.1) and nitrite reductase (NIR; EC 1.7.7.1) in the cotyledons of the mustard (Sinapis alba L.) seedling is strongly affected by externally supplied ammonium (NH2). In short-term experiments between 60 and 78 h aft
In mustard (Sinapis alba L.) cotyledons, NADH-dependent glutamate synthase (NADH-GOGAT, EC 1.4.1.14) is only detectable during early seedling development with a peak of enzyme activity occurring between 2 and 2.5 d after sowing. With the beginning of plastidogenesis at approximately 2 d after sowing