Physicochemical studies of biologically active peptides by low-temperature reversed-phase high-performance liquid chromatography
β Scribed by David E. Henderson; Jill A. Mello
- Book ID
- 104144961
- Publisher
- Elsevier Science
- Year
- 1990
- Tongue
- English
- Weight
- 542 KB
- Volume
- 499
- Category
- Article
- ISSN
- 1873-3778
No coin nor oath required. For personal study only.
π SIMILAR VOLUMES
## Abstract The low temperature effect has been investigated in reversed phase micro high performance liquid chromatography with various aqueous mobile phase systems by an approach based on enthalpyβentropy compensation. The compensation temperatures, Tc, were determined for these systems, and the
## Factors controlling the performance of peptide mapping on reversed-phase columns were systematically evaluated. Performance criteria included resolution (peak capacity and selectivity), system reproducibility, sensitivity and analysis speed. Column configuration, characteristics of packing mate