The permeation behavior of 3H-vidarabine (3H-9-P-D-arabinofuranosyladenine) and 14C-n-pentanol through different strata of hairless mouse skin was studied using a diffusion cell at 37O under steady-state conditions. Partition coefficients for the skin components versus 0.9% aqueous NaCl solution also were obtained. Various skin preparations including full-thickness skin, cellophane-stripped skin, and dermis membranes of different thicknesses were employed. The dermis membranes were considered to be diffusionally homogeneous, and the product of the permeability coefficient and the thickness was taken as the apparent diffusivity. The apparent diffusivities for both compounds investigated were independent of thickness. Therefore, it was concluded that the molecular diffusivity is constant throughout the dermis. Comparisons of permeability coefficients in various strata of the skin revealed that, while the stratum corneum is the major diffusional barrier, the epidermis appears to be significantly less permeable than the dermis.
Keyphrases 0 Vidarabine valerate prodrug-topical dosage forms, permeability differences through various mouse skin strata 0 Prodrugs, topical-based on simultaneous transport and bioconversion of vidarabine, permeability differences in various components of mouse skin Diffusivity-vidarabine and n-pentanol, effect of skin strata and thickness on diffusivity 0 Models, physical-based on simultaneous transport and bioconversion, effect of homogeneous distribution on prodrug evaluation